(3a) A strain of F+ E. coli cells that are chloramphenicol-resistant were mixed with F- E. coli cells with ampicillin-resistance gene. The mixture of bacteria was then plated onto an agar plate with
ampicillin chloramphenicol antibiotics. The colonies of bacteria that survived were then replica plated (transfer of microorganism colonies in the same spatial arrangement from a master plate onto another agar plate) onto an agar plate with
chloramphenicol ampicillin antibiotics. It was found that there exist bacterial colonies that are resistant to both chloramphenicol and ampicillin antibiotics.
To help you with this question, refer to the following diagrams:
Replica plating technique
Replica plating tool
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