Thursday, July 26, 2012

Section 7.3 DNA and Genomics Lecture notes (ERROR)

7.3.1  Sickle Cell anaemia

·         On chromosome 11, the 17th nucleotide of the gene that codes for the beta chain of haemoglobin is changed from an 'A' to a 'T'.  (GAG to GTG) à WRONG!!!

·         This changes the mRNA codon from 'CUC' to 'CAC' à WRONG!!!, resulting in the 6th amino acid of the chain being changed from glutamic acid to valine. The R side chain of glutamic acid is a polar whereas that of valine is hydrophobic. The mutant haemoglobin is known as Haemoglobin S (HbS).

·         This substitution of one single base in the beta globin gene alters the quaternary structure of haemoglobin, which has a profound influence on the physiology and wellbeing of the individual.

To change the above to:

7.3.1  Sickle Cell anaemia

·         On chromosome 11, the 17th nucleotide of the gene that codes for the beta chain of haemoglobin is changed from an 'T' to a 'A'. (template 3’ CTT 5’ to 3’ CAT 5’) à CORRECT

·         This changes the mRNA codon from 5' GAA 3' to 5’ GUA 3' à CORRECT, resulting in the 6th amino acid of the chain being changed from glutamic acid to valine. The R side chain of glutamic acid is a polar whereas that of valine is hydrophobic. The mutant haemoglobin is known as Haemoglobin S (HbS).

7 comments:

  1. Hello Miss Teong!

    May I know what's the difference between Okazaki Fragments and Lagging Strand? Or are they the same?

    Thanks! ;D

    ReplyDelete
    Replies
    1. Hi,

      It is not really the same thing. In a way, there are many okzaki fragments but only 1 lagging strand (that is synthesized discontinuously).

      All the okazaki fragments in totality will make up the one lagging strand.

      Ms Teong

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  2. Hi ! =D

    Why is there no need for a primer during transcription?

    Thank you!

    ReplyDelete
    Replies
    1. Only DNA Polymerase requires a primer!!

      Miss Teong

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    2. On pg 22 of lecture notes for DNA and Genomics,Fig6.1.3 shows a DNA of a prokaryote or a mRNA?

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    3. Is there a difference between mRNA and mRNA transcript?

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    4. 1) pg 22 shows a mRNA, as stated in the title clearly. why do u thk it is DNA?

      2) nope, mRNA is called a mRNA transcript becos it is formed after transcription.

      Ms Teong

      Delete